Fig. 5. Role of PKC and ROCK in 70K-induced contractions in rat basilar arterial rings. (A) Superimposed traces showing the effects of fasudil (1 mM) alone or fasudil+chelerythrine (5 mM) (gray) on the tonic component of the depolarization-induced contraction. (B) Representative trace showing the effect of PBDu (0.05 mM) on the vasorelaxant effect of fasudil in 70K-induced contractions. (C) Quantitative summary of the effects of fasudil, fasudil + chelerytrine and fasudil + PDBu on the tonic component of the depolarization-induced contractions (fasudil, n = 21; fasudil + chelerytrine, n = 6; fasudil + PDBu, n = 3). (D) Superimposed traces showing the isometric force developed in response to 70K in the presence of the ROCK inhibitor Y27632 (1 mM) alone or combined with GFX (0.5 mM) (gray). (E) Quantitative summary of the effect of GFX and Y27632 on the sustained component of the depolarization-induced contractions (Y27632, n = 3; Y27632 + GFX, n = 4). Statistical significance was calculated by Kruskal-Wallis test followed by Mann-Whitney U test. *P<0.05 vs. 70K, ^#P<0.05 vs. fasudil for panel C; *P<0.05 vs. control, ^#P<0.05 vs. Y27632 for panel (E).